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KMID : 1101320070390030190
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Korean Journal of Clinical Laboratory Science
2007 Volume.39 No. 3 p.190 ~ p.195
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The Cytotoxicity of Rat Hepatocytes on Viscum album Lectin
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Chang Choul-Soo
Ryu Jae-Ki
Kim Tae-Ho
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Abstract
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In the present study, the author investigated to the cytotoxocity in cultured rat hepatocytes of Viscum album lectin. The cytotoxcity effect in Viscum album lectin on the activity of LDH was also investigated. Viscum album lectin significantly increased LDH leakage into medium of hepatocytes treated or untreated with CCl4 (p<0.001). However, Viscum album lectin significantly increased LDH leakage from CCl4-induced hepatocyte (p<0.001). There was a significant increase in LDH levels relative to the control group. Histological observation basically supported the result obtained from LDH assay. The livers of rats challenged with CCl4 produced a marked increased cytoplasmic vacuoles and inflammatory cells in number, while the number of necrotic cells and swollen hepatocytes did not change significnatly. Rats administered DMSO alone did not alter the normal hepatic architecture. Histological observation of liver section in rat treated 72 hrs with either Viscum album lectin CCl4-induced liver damage showed number of cytoplasmic vaculoe and necrotic cell. The number of inflammatory cell increased markedly. This results suggest to the conclusion that Viscum album lectin has a effect of hepatotoxicity activator.
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KEYWORD
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Viscum album lectin, Cytotoxocity, Cultured rat hepatocytes
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